Recommended Applications

• Shampoo
• Conditioner
• Leave-on Products
• Styling Products

Keratin is a protein located in the hair cortex, consisting of long chains of amino acids. Thanks to the various sulfur atoms, the keratin chains coil into a helix-like structure. These bonds, called disulfide bridges, provide strength and resilience to the hair.

TECHNOLOGY

AmminexPro features a polymer matrix designed for the molecular reconstruction of damaged hair, containing medium molecular weight Hyaluronic Acid, Silk Proteins, Keratin, Hydrolyzed Elastin, Arginine, Glutamic Acid, and Lysine.

The synergistic effect of hydration and reconstruction derived from this polymer matrix combines with the reparative properties of essential amino acids for hair beauty.

AmminexPro penetrates the damaged cortex, repairing and reconstructing the hair from within, strengthening and making the cuticle compact and elastic.

Efficacy Assessment
IN VITRO ASSESSMENT OF RESTRUCTURING ACTIVITY

Standard human hair strands were subjected to damaging treatment under controlled conditions. Subsequently, they were treated with the tested product, analyzed using Scanning Electron Microscopy (SEM), and compared with untreated hair strands used as controls.

Pre-treatment of Samples

During the pre-treatment phase, brown hair strands were subjected to bleaching treatment to damage both the cuticle and cortex of the hair. Subsequently, each strand was washed with a neutral shampoo, treated with 0.1 ml of the sample, and dried.

Experimental Setup:

• One strand of bleached hair was treated with 0.1 ml of 5% aqueous solution of AmminexPro and dried after 5 minutes.
• Another strand of bleached hair, washed and dried, was used as the control.

Scanning Electron Microscopy (SEM)

SEM analysis of the hair is a useful test to photograph its morphology, a characteristic associated with the hair’s good health.

Healthy hair has a regular cuticle architecture, while damaged hair shows raised or, in the worst cases, broken cuticle scales with clusters of material attached to structural fragments of the hair.

In this test, samples were taken from each hair strand under examination and analyzed using Scanning Electron Microscopy (SEM) to assess the restructuring of the cortex and cuticle.

Specifically, the following SEM analyses were conducted:

• SEM on longitudinal hair sections to assess the cuticle’s condition and hair structure uniformity.
• SEM on transverse hair sections to examine the hair’s filling consistency.

AmminexPro forms a thin protective film that closes the cuticle scales.

In treated hair, an increased substantivity of the hair structure and evident repair were observed.

The surface of the hair used as a control shows an uneven texture with signs of fibrillation and non-overlapping cuticle scales (figure 1.a).

The specific spatial adaptation of the cuticle cells in the control is quite evident, even when the hairs are analyzed in transverse sections (figure 2.a and 3.a).

The following SEM images were taken on longitudinal hair sections of the examined hair (Figure 1):

Figure 1. Longitudinal section of the hair under examination

The SEM images were also captured on transverse hair sections of the examined hair (Figure 2 and Figure 3):

Figure 2. Transverse section of the hair under examination

Figure 3. Transverse section of the hair under examination immersed in liquid nitrogen

The hair treated with AmminexPro shows a significant improvement in the structure of the cuticle scales (Figure 1.b) and the hair cortex (Figure 2.b and Figure 3.b).

Compared to the control, the transverse sections display a greater continuity between the cuticle and the cortex. The compactness and uniformity of the hair structure are clearly evident.